ChIP-SEQ AND PEAK CALLING
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چکیده
et al details each of these steps and discusses how peak finding tools approach the separate steps very differently (3). A follow up review by Wilbanks et al evaluated the performance of 11 ChIP-seq peak callers nearly all of which are still widely used today (4). Each step can have parameters that can be adjusted by the user, but changing these can significantly affect the final peak lists. Care must be taken that data sets are analysed using the same methods. The ENCODE consortium produced guidelines for analysis of the dispersed data sets to avoid issues created by analysis parameter differences (5). This project used MACS, PeakSeq and SPP.
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تاریخ انتشار 2013